Expression of G1 cell cycle related genes in triamcinolone acetonide treated keloid fibroblasts.

Woo, Sang Hyun; Seul, Jung Hyun; Suh, Seong Il; Baek, Won Ki; Suh, Min Ho

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(2):178-190.
Published online February 1, 1998.

Expression of G1 cell cycle related genes in triamcinolone acetonide treated keloid fibroblasts.

Sang Hyun Woo, Jung Hyun Seul, Seong Il Suh, Won Ki Baek, Min Ho Suh

¹Department of Plastic Surgery, School of Medicine, Yeungnam University, Korea.
²Department of Microbiology, School of Medicine, Keimyung University, Taegu, Korea.

Abstract

The effect of triamcinolone acetonide(TA) on the expression of Gl related genes was investigated the cultured keloid fibroblast. The addition of TA to the culture medium resulted in growth inhibition of keloid fibroblast. TA reduced the expression of cyclin A, B, E and cyclin dependent kinase(CDK) 2 mRNA, but unexpectedly, the expression of cyclin C, Dl and CDK4 mRAN was not affected significantly as compared with those of normal fibroblast. Expressions of p16, p21 and p27, the wellestabilished CDK-inhibitors, were also investigated. The level of p16 was not detected in both normal and keloid fibroblasts and the expression of p27 was significantly decreased in keloid fibroblast. The expression of p21 was dramatically increased in keloid fibroblast but not significantly changed in normal fibroblast. Also the expressions of p53 and pRb, the well known tumor suppressor genes, were increased by the addition of TA. These data suggested that the observed growth inhibitory effect of TA may be related to transcriptional inactivation of cyclin A, B, E and CDK2 and to the transcriptional activation of p21, but the mechanisms of unchanged expression of cyclin C, Dl and CDK4 mRNA remain to be elucidated.

Keywords: Keloid; Triamcinolone acetonide; Cell cycle