J Korean Soc Plast Reconstr Surg Search

CLOSE


Journal of the Korean Society of Plastic and Reconstructive Surgeons 2001;28(1):26-31.
Published online January 1, 2001.
The Effect of Glucose Control on DNA and Collagen Synthesis of Cultured Fibroblasts of Chronic Diabetic Wounds.
Jae Deok Kim, Nak Heon Kang, Ho Kwon, Poong Lim, Goo Taeg Oh
1Kim Jae Deok Plastic and Aesthetic Surgical Clinic.
2Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea.
3Korea Research Institute of Bioscience and Biotechnology.
Abstract
Diabetes is accompanied by delayed wound healing and insufficient granulation tissue formation, possibly because of a defect in fibroblast function. Maintaining glucose level at acceptable low level is considered to be an important part of the clinical treatment of diabetes, but the exact mechanism by which diabetes delays wound repair is not yet known. This study was designed to evaluate the effects of glucose on proliferation([3H]-thymidine uptake) and collagen synthesis([3H]-proline uptake) in dermal fibroblasts from non-lesional skins and chronic wounds. The subjects were divided into 3 groups: non-diabetic control(n = 5), glucose controlled(glycated Hb < 7%) diabetics(n = 5), and glucose uncontrolled(glycosylated Hb > 8%) diabetics(n = 5). The fibroblasts were cultured in two conditions, low glucose(5 mM) and high glucose(20 mM) medium. The data were compared using Mann-Whitney test. The results are as follows: 1. There were no significant differences in the proliferation and collagen synthesis of non-lesional skin fibroblasts in all groups. 2. In high glucose medium culture, the proliferation of the fibroblasts from the wound of the glucose uncontrolled diabetic group was significantly lower than those of the other 2 groups(p< 0.05), and the collagen synthesis of the wound fibroblasts of the control group was significantly higher than those of 2 diabetic groups(p< 0.05). 3. The proliferation and collagen synthesis of the wound fibroblasts in all groups were significantly lower than those of the non-lesional skin fibroblasts(p< 0.05). 4. In the control group and the glucose controlled diabetic group, the proliferation of the wound fibroblasts in the high glucose culture media were lower than those in the low glucose culture media(p< 0.05). Also, in the glucose controlled diabetic group, the collagen synthesis of the wound fibroblasts in the high glucose culture medium was lower than that in the low glucose culture medium(p< 0.05). These results demonstrate that the glucose level may affect the proliferation and collagen synthesis of fibroblasts, and the strict blood glucose control is beneficial to the prevention and treatment of diabetic chronic wounds.
Keywords: Diabetes; Chronic wound; Fibroblast
TOOLS
Share :
Facebook Twitter Linked In Google+ Line it
METRICS Graph View
  • 132 View
  • 0 Download

Effect of Lipoaspirate Cell Autograft on Proliferation and Collagen Synthesis of Diabetic Fibroblasts in Vitro.2009 November;36(6)

The Effects of Verapamil on Growth and Apoptosis of Keloid Fibroblast.2005 September;32(5)

Effect of Oncostatin M on Proliferation and Matrix Synthesis of Dermal Fibroblasts.2008 March;35(2)

The Effect of Granulocyte-Macrophage Colony-Stimulating Factor on Human Dermal Fibroblast Proliferation.2001 November;28(6)

Comparative study of the Characteristics of Collagen Synthesis by Cultured Fibroblasts in the Fetus and Adult.1999 May;26(3)



ABOUT
ARTICLE & SPECIALITY
Article category

Browse all articles >

Speciality

Browse all articles >

BROWSE ARTICLES
AUTHOR INFORMATION
Editorial Office
101-2003, Lotte Castle President, 109, Mapodaero, Mapo-gu, Seoul 04146, Korea
Tel: +82-2-3472-8252    Fax: +82-2-3472-4254    E-mail: apsedit@gmail.com                

Copyright © 2019 by Korean Society of Plastic and Reconstructive Surgeons. All rights reserved.

Developed in M2community

Close layer
prev next