In vitro Fusion of the Posterior Frontal Calvarial Suture in the Mouse.

Roh, Tai Suk; Rah, Dong Kyun; Park, Chul; Park, Beyoung Yun

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2000;27(6):670-674.
Published online November 1, 2000.

In vitro Fusion of the Posterior Frontal Calvarial Suture in the Mouse.

Tai Suk Roh, Dong Kyun Rah, Chul Park, Beyoung Yun Park

¹Department of Plastic & Reconstructive Surgery, Yongdong Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. rohts@yumc.yonsei.ac.kr
²Yonsei University Institute for Human Tissue Restoration.

Abstract

The mechanism underlying cranial suture fusion remains yet unknown. An in vitro tissue culture model of cranial sutures permits exclusion of biomechanical factors that may affect the fusion and patency of cranial sutures. The posterior frontal cranial sutures were placed in a tissue culture model. The experimental groups consisted of control group, and two groups cultured in TGF-beta enriched medium with and without the underlying dura mater.

Results
showed in vitro fusion of the explanted cranial sutures in vitro 39 days postnatal. Cranial suture fusion was accelerated in the TGF- beta < treated group with intact dura mater(postnatal 32days). Cranial suture fusion in the TGF- beta treated dura denuded group occurred, though in a delayed manner compared to the dura intact group.

Keywords: Cranial suture; TGF beta; Dura mater; Suture fusion