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Journal of the Korean Society of Plastic and Reconstructive Surgeons 2000;27(3):308-313.
Published online May 1, 2000.
Effect of Platelet Supernatants on the Growth of Auricular Chondrocytes of Rabbit in Primary Culture.
Sung Yurl Yang
Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Abstract
The availability of a large number of chondrocytes is important for cartilage tissue engineering. Chondrocytes have been shown to be sensitive to the proliferative action of a wide variety of growth factors. Many of these growth factors have been isolated from platelets. In this study, we tested whether human platelet supernatants, as a supplement to basic medium, would support the proliferative and secretary activity of rabbit auricular chondrocytes in low- density monolayer culture. In 5% serum supplemented cultures, uptake of [H]-thymidine was increased in platelet supernatant-treated chondrocytes by 1.9-2.5 fold at 72 hours compared with controls. In serum free cultures, the ability of platelet supernatants to promote proliferative activity was decreased compared with serum supplemented cultures. In 5% serum supplemented cultures, glycosaminoglycan synthesis was increased in platelet supernatant-treated chondrocytes at 96 hours compared with controls. In serum supplemented cultures, seeding efficiency was increased in platelet supernatant-treated cultures by more than 3-fold compared with controls. These results indicate that platelet supernatant promotes proliferation and matrix synthesis of rabbit auricular chondrocytes. Platelet supernatants may be useful as a cheap autologous source of multiple growth factors to increase in vitro expansion of chondrocytes.
Keywords: Auricular chondrocytes; Platelet supernatants; Tissue engineering
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