AN immunoelectron microscopic study on the expression of fibronectin, type i collagen and type iii collagen during wound healing of rat skin.

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Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 1998;25(7):1185-1197.
Published online October 1, 1998.

AN immunoelectron microscopic study on the expression of fibronectin, type i collagen and type iii collagen during wound healing of rat skin.

No authors listed

Abstract

This study was focused on the localizations or expressions of fibronectin and interactions of fibronectin with type I and type III collagen in the new forming tissues during wound healing in rat skin. Adult male rats(SPrague-Dawley strain), 150-200 gm weight, were used for experimental animals. And the experimental animals were divided into 3 or 4 groups by passed days after incision of back skin.The specimens were obtained and immunohistologically stained for electron microscopy from each of groups and the specimens were observed with the electron microscope (Hitach-600 Model). The results obtained were as follows. 1. Strong positive reactions for type I collagen are seen in the new forming tissue at day 3 after incision of skin, and moderate reactions at day 5 and day 7 after incision of skin. 2. The weak reactions for type III collagen are seen at day 3 and day 5 after incision in the new forming tissues of skin. And the type III collagen reaction at day 7 are more increased than that of other groups. 3. Fibronectin expressions are seen moderately at day 5 and day 3 groups, and strong at day 5 group, but at day 7 group reactions are rapidly decreased. It is suggested that type I and III collagens are gradually increased from the beginning of wound healing to the time of the new fibrous tissues formation. The great activities of the fibronection are seen within processes of the new tissue formation for wound healing events.