Gene Expression Profiling of 1,2-Dimethylhydrazine-Stimulated Human Umbilical Vein Endothelial Cells.

Kim, Hyun Ok; Kang, Young Seok; Bae, Yong Chan; Park, Suk Young; Hwang, So Min; Nam, Su Bong

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Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2004;31(6):858-864.
Published online November 1, 2004.

Gene Expression Profiling of 1,2-Dimethylhydrazine-Stimulated Human Umbilical Vein Endothelial Cells.

Hyun Ok Kim, Young Seok Kang, Yong Chan Bae, Suk Young Park, So Min Hwang, Su Bong Nam

¹ABC Aesthetic Clinic, Busan, Korea.
²Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University, Busan, Korea.

Abstract

The purposes of this study are to establish standard model in which endothelial cell proliferations are induced by DMH stimulation in vitro, and to analyze the gene expressions of proliferative HUVECs using DNA chip technique which could evaluate the mechanisms of angiogenesis, and the development of vascular tumors. To perform the MTT assay in 96-well microplates, 104 cells were seeded in each well which were cultured in medium. On the third day, the cells were treated with 5 different concentrations of diluted DMH from 10 to 10-3 ng/ml. Five DMH-treated groups were compared with the control group which was not treated with DMH. The optical densities in each group were measured at the time of 0, 6, 12, 24, 36, 48, and 72 hours after DMH treatment. The same experiment was repeated 9 times. Statistically significant cell proliferations were observed in 1 and 10-1ng/ml group. The RNAs were isolated from HUVECs of control group and 1ng/ml DMH-treated group, and they were used to analyze the gene expressions using DNA chip technique. One hundred and seventy-seven genes(142 of up-retulated genes and 35 down-regulated genes) were identified, and several genes were associated with VEGF and FGF production. Also DMH could affect expression of genes that involve oncogenesis. Further study should be performed to evaluate the processes of angiogenesis and morphogenesis of vascular tumors, which could be utilized in the development of new therapeutic approaches.

Keywords: Dimethylhydrazines; Vascular endothelial cells; Umbilical vein; DNA chip; Gene expression

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