Influence of Keloid-derived Keratinocyte on TGF- beta1 Production of Fibroblast in Co-culture Model.

Park, Jun; Kang, Sang Yoon; Yoo, Young Cheun; Yang, Won Yong

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2004;31(4):554-562.
Published online July 1, 2004.

Influence of Keloid-derived Keratinocyte on TGF- beta1 Production of Fibroblast in Co-culture Model.

Jun Park, Sang Yoon Kang, Young Cheun Yoo, Won Yong Yang

Department of Plastic and Reconstructive Surgery, College of Medicine, Kyung Hee University, Seoul, Korea.

Abstract

Keloids represent a pathological response to injury interrupting skin integrity, creating disfiguring scars with no definitive pathophysiology. Transforming growth factor-beta1(TGF- beta1) has been regarded as one of the pathogenesis of keloids. The purpose of this study is to investigate the influence of keloid keratinocytes on TGF-beta1 expression in co-culture system. Recent studies have highlighted the important concept of epithelial- mesenchymal interactions in normal skin biology, and applying this concept to keloids in vitro studies demonstrated significantly increased proliferation of fibroblasts and collagen production in keloid fibroblasts co-cultured with keloid-derived keratinocytes as compared with normal keratinocytes. In this study, normal or keloid fibroblasts in lower chamber were co-cultured with keratinocytes derived either from normal skin or keloid tissue in upper chamber, separated from lower chamber by permeable membrane.

Results
obtained by ELISA showed equal TGF-beta1 expression when keloid fibroblasts were co- cultured with keloid keratinocytes, as compared with the normal keratinocytes at 24,48 hours, but significantly higher expression at 72 hours(p=0.0358). RT-PCR demonstrated that the TGF- beta1 expression level was not significantly related to the level of mRNA encoding TGF-beta1. These results suggest that overlying keloid keratinocytes play an important role in inducing high level of TGF- beta1 expression in keloid by paracrine effect or epithelial-mesenchymal interaction.

Keywords: Keloid; TGF-beta1