J Korean Soc Plast Reconstr Surg Search

CLOSE


Journal of the Korean Society of Plastic and Reconstructive Surgeons 2004;31(1):89-94.
Published online January 1, 2004.
The Effects of Growth Factors on Motility of Cultured Human Dermal Microvascular Endothelial Cell.
Won Jai Lee, Young Soo Kim, Jong Chul Park, Bong Joo Park, Beyoung Yun Park, Dong Kyun Rah
1Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Korea. dkrah@yumc.yonsei.ac.kr
2Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
Abstract
Cell migration is essential for many important biological events, including embryonic development, wound healing, inflammatory response, and tumor metastasis. As a result of endothelial cell migration, angiogenesis is very important factor in embryogenesis, wound healing, tumor development and flap survival. Angiogenesis is dependent on endothelial cell proliferation, migration and motility is one of the most essential for many important biological events. The speed of cell migration is regulated by extension, attachment, detachment of cell membrane and adhesiveness of cell to extracellular matrix. Growth factors such as FGF, TGF, VEGF is well known to play a major roles in the migration of endothealial cells. This study was designed to compare the motilities of human dermal microvascular endothelial cell(HDMEC) in growth factors such as bFGF, TGF-beta1 and VEGF. The motility of cultured HDMEC was compared using a video-microscopy system that was developed in combination with a self-designed CO2 mini- incubator. To determine migration speed, cells were viewed with a 4 phase-contrast lens and video recored. Images were captured using a color CCD camera and saved in 8-bit full-color mode. Experimental groups were divided into four groups: group I(with a Control, HDMEC only), group II(HDMEC with bFGF), group III (HDMEC with TGF-beta1), group IV(HDMEC with VEGF). At the concentration of 1ng/ml(bFGF), 1ng/ml(TGF-beta1), and 10ng/ml(VEGF) as the most effective dose for cell migration through preliminary study, the speed of migration are 8.736+/-0.948micrometer/hr, 9.869+/-1.904micrometer/ hr, 10.293+/-1.612micrometer/hr, respectively. These data shows that groups with growth factor accelerate the HDMEC migration than a control group, and the VEGF is most effective growth factor in the HDMEC migration than bFGF and TGF-beta1.
Keywords: HDMEC; Cell migration; bFGF; TGF-beta1; VEGF
TOOLS
Share :
Facebook Twitter Linked In Google+ Line it
METRICS Graph View
  • 153 View
  • 0 Download

The Effect of Basic Fibroblast Growth Factor in Acellular Human Dermal Grafts in Rats.2011 September;38(5)

Effect of Hypoxia and Reoxygenation on Cultured Human Dermal Fetal Fibroblast.2005 May;32(3)

Activity of Protein Kinase C in Abnormally Proliferated Vascular Endothelial Cells.2007 January;34(1)

Effect of Progesterone on Cultured Human Dermal Fibroblast.2007 July;34(4)

The Effect of Radiation on the Patency of End-to-side Microvascular Anastomosis.2001 September;28(5)



ABOUT
ARTICLE & SPECIALITY
Article category

Browse all articles >

Speciality

Browse all articles >

BROWSE ARTICLES
AUTHOR INFORMATION
Editorial Office
101-2003, Lotte Castle President, 109, Mapodaero, Mapo-gu, Seoul 04146, Korea
Tel: +82-2-3472-8252    Fax: +82-2-3472-4254    E-mail: apsedit@gmail.com                

Copyright © 2019 by Korean Society of Plastic and Reconstructive Surgeons. All rights reserved.

Developed in M2community

Close layer
prev next