Tissue-Engineered Human Neocartilage Formation Using Human Adult Auricular Cell Line.

Chung, Jai ho; Kim, Seung Up; Park, Beyoung Yun; Park, Myong Chul; Yim, Hyunee; Lee, Myung Ae; Hong, Hyun Seok

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Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2003;30(5):639-647.
Published online September 1, 2003.

Tissue-Engineered Human Neocartilage Formation Using Human Adult Auricular Cell Line.

Jai ho Chung, Seung Up Kim, Beyoung Yun Park, Myong Chul Park, Hyunee Yim, Myung Ae Lee, Hyun Seok Hong

¹Department of Plastic & Reconstructive Surgery, Ajou University School of Medicine, Suwon, Korea. jhchung@ajou.ac.kr
²Department of Brain Disease Research Center, Ajou University School of Medicine, Suwon, Korea.
³Institute for Human Tissue Restoration, Department of Plastic & Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Korea.
⁴Department of Pathology, Ajou University School of Medicine, Suwon, Korea.

Abstract

The purpose of these studies was an establishment of human auricular chondrocyte cell line using retrovirus mediated v-myc transfer, characterizing the human auricular chondrocyte cell line by type II collagen mRNA expression and transplantation of human auricular cell line into immunological incompetent nude mice to establish neocartilage formation. Also, I evaluated the growth rate of chondrocyte cell line to measure the cellular proliferative potency. I have established the human auricular chondrocyte cell line integrated v-myc and confirmed by v-myc transduced Myc protein expression by immunohistochemistry and immunoblotting study. And, growth rate of established human auricular chondrocyte cell line increased 4 folds times faster than primarily cultured human auricular chondrocyte. The established human auricular chondrocyte had type II collagen mRNA upto 8 months in monolayer culture. And we observed formation of neocartilage on the back of nude mice using chondrocyte cell line/fibrin glue polymer at 12 weeks transplantation.

Keywords: Chondrocyte cell line; v-myc; Collagen type II; Neocartilage

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