Preadipocyte Culture in Chitosan-Alginate Gel.

Kwon, Ho; Yoon, In Mo; Seok, Yoon; Cho, Hyun Mi; Rhie, Jong Won; Ahn, Sang Tae

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2003;30(5):635-638.
Published online September 1, 2003.

Preadipocyte Culture in Chitosan-Alginate Gel.

Ho Kwon, In Mo Yoon, Yoon Seok, Hyun Mi Cho, Jong Won Rhie, Sang Tae Ahn

Department of Plastic Surgery, The Catholic University of Korea College of Medicine, Seoul, Korea. rhie@catholic.ac.kr

Abstract

Alginate gel is widely used as a scaffold in tissue engineering. Alginate solution has anionic properties, and calcium or magnesium cation has been used to crosslink alginate into a gel form. Chitosan not only has cationic properties but also is known to promote wound healing. Although there are some studies of chitosan- alginate gel use in drug delivery, reports of its application as a scaffold in tissue engineering are rare. The purpose of this study is to make chitosan-alginate gel and to investigate its biocompatibility as a scaffold for preadipocyte culture. 1, 2, 4, 6% chitosan solutions were mixed with 2% alginate solution to make various concentrations of chitosan-alginate gels. All of the gel which were made have been measured by Viscometer. Preadipocytes obtained from human breast fat tissue were seeded into each chitosan-alginate gel, and cell viability was measured by XTT colorimetric assay on the 2th, 4th, and 7th day of preadipocyte culture. The results of analysis were as follows. Each viscosity of 4% and 6% chitosan-alginate gels is similar to that of the calcium-alginate gel and 4% and 6% chitosan-alginate gels shows significantly higher cell viability than the calcium-alginate gel(p<0.05). In conclusion, chitosan-alginate gel is thought to be an appropriate scaffold for preadipocyte culture in tissue engineering.