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Journal of the Korean Society of Plastic and Reconstructive Surgeons 2003;30(5):628-634.
Published online September 1, 2003.
Expression of mRNA for Monocyte Chemoattractant Protein-1 in the Sciatic Nerve and Hindlimb Muscle Following Denervation.
Jeong Hoon Song, Young Cheon Na, Myoung Ae Choi, Min Sun Kim, Byung Rim Park
1Department of Plastic and Reconstructive Surgery, School of Medicine, Wonkwang University, Iksan, Korea. ycnadr@wmc.wonkwang.ac.kr
2Department of Physiology, School of Medicine, Wonkwang University, Iksan, Korea.
Abstract
Migration of inflammatory cells into damaged tissue is essential to host defense mechanisms and immune responses. Monocyte chemoattractant protein-1(MCP-1), a member of the CC or beta-chemokine family is a powerful leukocyte recruitment factor that is relatively specific for monocyte/macrophage. The purpose of present study was to evaluate temporal change of expression of mRNA for MCP-1 in the sciatic nerve and hindlimb muscle of Sprague-Dawley rat. The mid-portion of the sciatic nerve was exposed, cut under aseptic condition and then animals were sacrificed 1, 2, 3, 7, 14 days after denervation of the sciatic nerve. Semiquantitative RT-PCR method and image analysis system were used to analyze change in expression of mRNA for MCP-1. Expression of mRNA for MCP-1 in the distal stump of the denervated sciatic nerve was up-regulated within 1 day and peaked on the second day following transection of the sciatic nerve. Thereafter high expression of mRNA for MCP-1 lasted for 7 days after transection of the nerve. However the expression of mRNA for MCP-1 was rapidly decreased on the 14th day after denervation. Temporal change of expression of mRNA for MCP-1 in the proximal portion of the denervated sciatic nerve was very similar with that of distal one. The gastrocnemius muscle also showed significant increase in expression of mRNA for MCP-1 on the 1st day with maximal expression on the 7th day after denervation of the sciatic nerve. In contrast, the expression of mRNA for MCP-1 in the soleus muscle was lower than the gastrocnemius muscle in the course of the denervation-induced atrophy. Additionally alpha-lipoic acid, a potent antioxidant that is used in treatment for diabetic neuropathy, suppressed significantly expression of mRNA for MCP-1 in the denervated sciatic nerve but not in atrophied hindlimb muscles.
Keywords: MCP-1; Sciatic nerve; Hindlimb muscle; alpha-Lipoic acid
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