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Journal of the Korean Society of Plastic and Reconstructive Surgeons 2008;35(4):367-372.
Published online July 1, 2008.
The Effect of Donor Antigen-pulsed Dendritic Cells on Survival of Skin Allograft in a Rat Model.
Seok Chan Eun, Byung Jun Kim, Jin Hee Kim, Chan Yeong Heo, Rong Min Baek, Hak Chang, Kyung Won Minn
Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Gyeonggi, Korea. sceun@snubh.org
Abstract
PURPOSE
Prevention of acute rejection in skin allografts without continuous immunosuppression lacks reports in worldwide literature. Needs for chronic immunosuppression preclude the use of tissue allograft as a routine surgical reconstructive option. Recently dendritic cells(DC) gained considerable attention as antigen presenting cells that are also capable of immunologic tolerance induction. This study assesses the effects of alloantigen-pulsed dendritic cells in induction of survival increase in a rat skin allograft model. METHODS: Recipient-derived dendritic cells were harvested from rat whole blood and cultured with GM- CSF(200ng/mL) and IL-4(8ng/mL) for 2 weeks. Then donor-specific alloantigen pulsed dendritic cells were reinjected into tail vein before skin graft. The rat dorsal skin allografts were transplanted in 5 subgroups. Groups: I) untreated, II) anti-lymphocyte serum(ALS, 0.5 mL), III) FK-506(2mg/kg), IV) DCp, VI) DCp and FK- 506. Graft appearance challenges were assessed postoperatively.
RESULTS
The group V(DC and FK-506 treated) showed longest graft survival rate(23.5 days) than other groups; untreated(5.8 days), ALS(7.2 days), FK-506 (17.5 days), DCp(12.2 days).
CONCLUSION
Donor antigen pulsed host dendritic cell combined with short-term immunosuppression prolong skin allograft survival and has potential therapeutic application for induction of donor antigen specific tolerance.
Keywords: Skin allograft; Dendritic cell; Immune tolerance
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