Effect of Oncostatin M on Proliferation and Matrix Synthesis of Dermal Fibroblasts.

Chun, Kyung Wook; Lim, Hyung Woo; Han, Seung Kyu; Kim, Woo Kyung

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2008;35(2):115-120.
Published online March 1, 2008.

Effect of Oncostatin M on Proliferation and Matrix Synthesis of Dermal Fibroblasts.

Kyung Wook Chun, Hyung Woo Lim, Seung Kyu Han, Woo Kyung Kim

¹Department of Plastic Surgery, Dankook University, Korea.
²Department of Plastic Surgery, Korea University College of Medicine, Korea. pshan@kumc.or.kr

Abstract

PURPOSE
Oncostatin M(OSM) is a multifunctional cytokine that belongs to the interleukin(IL)-6 family. Although there have been a number of studies that focused on the role and mechanism of OSM in various organs and tissues, there are few reports on its effect on wound healing. The final purpose of this project is to evaluate the effect of OSM on wound healing. This pilot study was designed to investigate the effect of OSM on proliferation and matrix synthesis of human dermal fibroblasts, which are the major components of the wound healing. METHODS: Excess skin that was obtained from patients who underwent skin grafts, was used for this study. From this material, fibroblasts were isolated and cultured. The cultured fibroblasts were treated with one of four concentrations of OSM. The OSM concentrations used were 0, 50, 100, and 200ng/ml, respectively. After the OSM treatment, cell proliferation was determined by the MTT assay, collagen synthesis by the C1CP method, GAG levels by the Blyscan Dye method. The parameter levels of each group were compared.
RESULTS
OSM treatment increased all the components tested in the study. In particular, cell proliferation, GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U-test). The highest increase in all the components was obtained at a 100ng/ml concentration of OSM.
CONCLUSION
The results of the present study indicate that OSM stimulates proliferation and matrix synthesis of human dermal fibroblast and the optimal concentration for wound healing is 100ng/mL.

Keywords: Oncostatin M; Dermal fibroblasts