Comparison of Dipogenic and Osteogenic Potentials of Visceral and Subcutaneous Adipose Stem Cells of Human.

Kang, Min Gu; Park, Jong Lim; Lee, Jin Hee; Chang, Hak; Minn, Kyung Won; Park, Gyu Ju

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2007;34(5):537-542.
Published online September 1, 2007.

Comparison of Dipogenic and Osteogenic Potentials of Visceral and Subcutaneous Adipose Stem Cells of Human.

Min Gu Kang, Jong Lim Park, Jin Hee Lee, Hak Chang, Kyung Won Minn, Gyu Ju Park

¹Department of Plastic and Reconstructive Surgery, University of Ulsan College of Medicine, Ulsan, Korea. kmgu@paran.com
²Department of Plastic and Reconstructive Surgery, Reserch Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Korea.
³Department of Surgery, Seoul National University College of Medicine, Seoul, Korea.

Abstract

PURPOSE
Adipose tissue contains a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. It is well known that fat depots from different part of our body shows different nature not only in morphological aspect but also physiologic aspect. The authors compared the adipogenic potentials and osteogenic potentials of adipose stem cells from different anatomical sites of human. METHODS: After laparotomy by surgery team, the authors isolated these adipose stem cells successfully from 7 men with an average age of 58, and induced differentiation along adipogenic and osteogenic lineages in vitro. On the 14th day, cells cultured in adipogenic media differentiated into adipocytes in vitro, as evidenced by positive Oil Red O staining of lipid vacuoles. On the 21st day, cells cultured in osteogenic media differentiated into osteoblasts in vitro as demonstrated by Alizarin red staining of a calcified extracellular matrix.
RESULTS
After exposure to adipogenic and osteogenic differentiation medium, subcutaneous adipose stem cells were found to possess greater adipogenic and osteogenic potentials than cells isolated from visceral adipose tissues.
CONCLUSION
This study indicates that adipogenic and osteogenic potentials of adipose stem cells vary by their anatomical sites, with subcutaneous adipose stem cells exhibiting higher adipogenic and osteogenic potential than those isolated from visceral fat.

Keywords: Adipose stem cells; Adipogenic and osteogenic potentials