Effect of Progesterone on Cultured Human Dermal Fibroblast.

Kwon, Soon Sung; Oh, Myung June; Lee, Jin Hee; Park, Jong Lim; Chang, Hak; Minn, Kyung Won

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2007;34(4):420-425.
Published online July 1, 2007.

Effect of Progesterone on Cultured Human Dermal Fibroblast.

Soon Sung Kwon, Myung June Oh, Jin Hee Lee, Jong Lim Park, Hak Chang, Kyung Won Minn

¹Research Institute of Plastic and Reconstructive Surgery, Seoul National University, Korea.
²Department of Plastic and Reconstructive Surgery, College of Medicine, Inha University, Incheon, Korea. frc22@hanmail.net
³Department of Plastic and Reconstructive Surgery, College of Medicine, Seoul National University, Korea.
⁴Department of Plastic and Reconstructive Surgery, Boramea Hospital, Seoul, Korea.

Abstract

PURPOSE
The mechanism of scar formation is not fully understood. Fibroblast is an important cell in wound healing process. We experienced a patient who was taking progesterone orally. Upper blepharoplasty was performed on her but, wound healing was delayed. We hypothesized that progesterone was the cause of delayed wound healing and fibroblast proliferation inhibition. We investigated the effect of progesterone in vitro on human dermal fibroblasts to study the effects on fibroblast proliferation. METHODS: Human dermal fibroblasts from four persons were cultured initially. Progesterone is mixed to them at various concentrations, and fibroblast cell count was measured by MTT assay method at 570nm. We confirmed that progesterone has some inhibitory effect on fibroblast proliferation and maximal inhibitory concentration of progesterone was determined. Then fibroblasts from a total of nineteen persons were cultured and the effects of progesterone were studied.
RESULTS
The initial study showed the maximal inhibitory concentration of progesterone to be 50 microgram/ml. The main study showed that progesterone had 70.9% inhibitory effect on human dermal fibroblast in vitro.
CONCLUSION
Progesterone has inhibitory effect on cultured human dermal fibroblast proliferation in vitro.

Keywords: Progesterone; Fibroblast