The Effects of the Mixture of Fetal Bovine Serum and Poly-glycolic acid in Rabbit Calvarial Model.

Sung, Yong Duck; Kim, Yong Ha; Moon, Young Mi; Kim, Kap Joong; Kim, Yeon Jung; Choi, Sik Young

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Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2007;34(3):298-304.
Published online May 1, 2007.

The Effects of the Mixture of Fetal Bovine Serum and Poly-glycolic acid in Rabbit Calvarial Model.

Yong Duck Sung, Yong Ha Kim, Young Mi Moon, Kap Joong Kim, Yeon Jung Kim, Sik Young Choi

¹Department of Plastic & Reconstructive Surgery, College of Medicine, Yeungnam University, Daegu, Korea. kimyon@yumail.ac.kr
²Tissue Engineering, College of Medicine, Yeungnam University, Daegu, Korea.
³Applied Chemistry, Natural Science, Andong National University, Andong, Korea.

Abstract

PURPOSE
This study was undertaken to investigate the osteogenic induction potential of PGA & FBS mixture on a calvarial defect in the rabbit. METHODS: Twenty New zealand white rabbit, weighing from 3.5-4kg were allocated into each of the three groups. Four 8mm sized bone defects were made on the parietal bone by drilling. In group I, the bony defects were implanted with 50 micrometer thickness film containing mixture of PGA and FBS. In group II, with PGA only film, & in group III, the bony defects were left with no implants.

Results
were evaluated by using morphologic change, radiographic study, biochemical study and histologic examination at 1 week (group I n=7, group II n=7, group III n=14), 2 weeks (group I n=6, group II n=6, group III n=12) and 3 weeks (group I n=7, group II n=7, group III n=14) following implantation.
RESULTS
In the morphologic & radiographic study, the formation and corticalization of callus were observed earlier in group I than in groups II and III (p<0.05). In histological examination, group I showed more abundant and faster new bone formation than in group II and III. In biochemical analysis, group I displayed more activity than in group II and III. Group I also showed more abundant osteopontin, osteocalcin than groups II and III.
CONCLUSION
In conclusion, the results demonstrate that the mixture of PGA and FBS has an effect on osteoblastic formation in the rabbit model. It is considered that further evaluation of long term results on resorption, immunologic tissue reaction and response of applied mixture in the human model will be needed.

Keywords: Fetal bovine serum; Poly-glycolic acid; Rabbit model

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