The Expression of CD 18 on Ischemia-Reperfusion Injury of TRAM Flap of Rats.

Yoon, Sang Yub; Lee, Taik Jong; Hong, Joon Pio

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2006;33(6):737-741.
Published online November 1, 2006.

The Expression of CD 18 on Ischemia-Reperfusion Injury of TRAM Flap of Rats.

Sang Yub Yoon, Taik Jong Lee, Joon Pio Hong

¹Baram Clinic BBC, Seoul, Korea. pssurgeon@korea.com
²Asan Medical Center, Department of Plastic Surgery, University of Ulsan, College of Medicine, Seoul, Korea.

Abstract

PURPOSE
This study was to evaluate the expression pattern of CD 18(leukocyte adhesion glycoprotein) in ischemia-reperfusion injury of TRAM flap of rats. Through this study, we can obtain more information about ischemia-reperfusion injury. We want to develop specific medicine to improve the survival rate of TRAM flap in the future. METHODS: A TRAM flap supplied by a single pedicle superior epigastric artery and vein was elevated on 60 Sprauge-Dawley rats. The rats were divide into 6 groups (each group n=10); Group O: sham, no ischemia-reperfusion injury, Group I: 2 hour reperfusion after 4 hour ischemia, Group II: 4 hour reperfusion after 4 hour ischemia, Group III: 8 hour reperfusion after 4 hour ischemia, Group IV: 12 hour reperfusion after 4 hour ischemia, and Group V: 24 hour reperfusion after 4 hour ischemia. This study consisted of gross examination for flap survival and flow cytometry study of CD18 on neutrophils.
RESULTS
The gross measurement of the flap showed different survival rate in group I(71%), II(68%), III(37%), IV(34%) and V(34%). All experimental groups showed an increase in the expression of CD18 compared to group O. The expression of CD18 was rapidly increased in ascending order in group I, II and III. But, the expression of CD18 was maintained in group IV and V.
CONCLUSION
The results can be implemented in the study to develop drugs which are capable of reducing ischemia-reperfusion injury in microsurgical breast reconstruction.

Keywords: Ischemia-Reperfusion Injury; CD18; TRAM flap