The Growth of Human Osteoblasts in Culture Dishes Made with Poly-glycolic Acid Containing Fetal Bovine Serum.

Choi, Jae Won; Kim, Yong Ha; Moon, Young Mi; Kim, Yoen Jung; Choi, Sik Young

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2006;33(5):612-615.
Published online September 1, 2006.

The Growth of Human Osteoblasts in Culture Dishes Made with Poly-glycolic Acid Containing Fetal Bovine Serum.

Jae Won Choi, Yong Ha Kim, Young Mi Moon, Yoen Jung Kim, Sik Young Choi

¹Department of Plastic & Reconstructive Surgery, College of Medicine, Yeungnam University, Daegu, Korea. yhkim@med.yu.ac.kr
²Department of Tissue Engineering, College of Medicine, Yeungnam University, Daegu, Korea.
³Applied Chemistry, Natural Science, Andong National University, Andong, Korea.

Abstract

PURPOSE
An ideal bony construct can be divided into two broad categories: (1) the design and fabrication of biodegradable, biomimetic scaffolds that provide correct signals to induce osteogenesis: (2) the identification of an ideal source of osteoprogenitor cells to seed onto the scaffold. We selected poly-glycolic acid as a synthetic scaffold among various scaffolds because of these properties. Meanwhile, culture medium is supplemented with fetal bovine serum(FBS): such serum contains essential elements such as proteins, hormones, growth factors and trace minerals. The composition of FBS can be ideal for various cell growth in vitro. We supposed that we could enhance bone growth at a fractured site if FBS was mixed with synthetic scaffold-PGA. METHODS: We cultured human osteoblasts in five different prepared culture dishes made with FBS and PGA mixture. The mixtures contained different ratio of FBS, that is, 0, 1.5, 3, 7, and 10%. We cultured human osteoblasts for seven days and examined the growth and attachment of the cells at the 1st, 3rd, 5th, 7th days, respectively.
RESULTS
In the mixture of 0% FBS and PGA, the growth of the cells lasted for one day. In 1.5 and 3% FBS and PGA, the growth of the cells was examined at the 3rd day, then minimally declined at the 5th and 7th days. In 7% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. In 10% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. Staining status of the osteoblasts with alkaline phosphatase showed pale pink color in 0% FBS and PGA groups, but bright pink color in 1.5, 3, 7, 10% FBS and PGA groups, especially in 3%, 7%.
CONCLUSION
In consequence, the growth of human osteoblast was higher in the mixture of FBS and PGA groups than in pure PGA ones. It is assumed that the mixture of FBS and PGA affects the proliferation of human osteoblasts.

Keywords: Fetal bovine serum; Poly-glycolic acid; Human Osteoblast