The Effect of Heparin on Gene Expression of Collagen, MMP, TGF-beta Isoforms Using Cultured Fibroblast from Transverse Carpal Ligament of Carpal Tunnel Syndrome.

Jeong, Seong Ho; Na, Min Hwa; Lee, Jae Sun; Song, Sang Hun; Yoon, Eul Sik; Dhong, Eun Sang; Kim, Han Joong

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2006;33(4):427-432.
Published online July 1, 2006.

The Effect of Heparin on Gene Expression of Collagen, MMP, TGF-beta Isoforms Using Cultured Fibroblast from Transverse Carpal Ligament of Carpal Tunnel Syndrome.

Seong Ho Jeong, Min Hwa Na, Jae Sun Lee, Sang Hun Song, Eul Sik Yoon, Eun Sang Dhong, Han Joong Kim

¹Department of Plastic and Renconstructive Surgery, Korea University College of Medicine, Seoul, Korea. prsdhong@kumc.or.kr
²Department of Plastic and Reconstructive Surgery, Ehwa Wemen's University College of Medicine, Seoul, Korea.

Abstract

PURPOSE
Noninflammatory synovial fibrosis has been noted for main causal factor of carpal tunnel syndrome (CTS). Recently, there are some reports that heparin have not only anti-coagulative effect but also anti-inflammatory and anti-fibrotic potential and have an effect on interstitial pulmonary fiborosis. Authors examined whether heparin affects pathogenesis of CTS. METHODS: First, heparin was administered to fibroblast that was cultured from patient's transverse carpal ligament. Secondly, we evaluated the expression from genes of type I, III collagen, TGF beta isoforms and MMP. Fibroblasts were isolated and cultured from transverse carpal ligaments of 5 patients with CTS. Heparin (0, 1, 10,100microgram/ml) was administered to cultured fibroblast and reverse transcription PCR for mRNA expression of type I, III collagen, TGF-beta isoforms and MMP was done.
RESULTS
Heparin suppressed gene expression of type I, III collagen and TGF-beta1, beta3 but promoted gene expression of TGF-beta(2) and MMP-2.
CONCLUSION
Heparin directly suppress gene expression of type I, III collagen. But, It is undetermined that heparin can present it's effect mediated by TGF beta iso forms or MMP.

Keywords: Heparin; Fibroblast; Collagen; MMP; TGF-beta