Effect of Hypoxia and Reoxygenation on Cultured Human Dermal Fetal Fibroblast.

Park, Beyoung Yun; Choi, Jong Woo; Kwark, Hyug Jun; Lee, Won Jai; Rah, Dong Kyun

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2005;32(3):347-356.
Published online May 1, 2005.

Effect of Hypoxia and Reoxygenation on Cultured Human Dermal Fetal Fibroblast.

Beyoung Yun Park, Jong Woo Choi, Hyug Jun Kwark, Won Jai Lee, Dong Kyun Rah

Institue for Human Tissue Restoration, Department of Plastic & Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Korea. dkrah@ yumc.yonsei.ac.kr

Abstract

In control group, fetal and adult fibroblasts were cultured under normoxic condition. The experimental groups were allocated into four different groups. The differences in TGF-beta, VEGF under 24, 48, 72 hours were statistically investigated. Compared to adult fibroblast group, there was a statistically significant increase (p<0.01) in the rates of protein synthesis in TGF-beta and VEGF of fetal fibroblast. In this study, these results may reflect the possibility that fetal fibroblast are more susceptible to change in oxygen and has a superior rate of angiogenesis through increased VEGF expression. The possible superiority of angiogenesis in fetal fibroblast may play an important role in scarless wound healing.

Keywords: Hypoxia; Re-oxygenation; Fetal fibroblast; Collagen; TGF-beta; VEGF