Wound Healing Effect of Bacterial Synthesized Cellulose on Full Thickness Skin Defect in the Rat.

Park, Sang Uk; Lee, Byung Kwon; Kim, Mi Sun; Park, Kwan Kyu; Sung, Woo Jung; Kim, Hyun Yeon; Kim, Chanwoo; Shim, Jeong Su; Lee, Yong Jig; Kim, Seong Ho; Kim, In Ho; Park, Dae Hwan

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2011;38(6):733-739.
Published online November 1, 2011.

Wound Healing Effect of Bacterial Synthesized Cellulose on Full Thickness Skin Defect in the Rat.

Sang Uk Park, Byung Kwon Lee, Mi Sun Kim, Kwan Kyu Park, Woo Jung Sung, Hyun Yeon Kim, Chanwoo Kim, Jeong Su Shim, Yong Jig Lee, Seong Ho Kim, In Ho Kim, Dae Hwan Park

¹Department of Plastic and Reconstructive Surgery, College of Medicine, Daegu Catholic University, Daegu, Korea. dhpark@cu.ac.kr
²Department of Pathology, College of Medicine, Daegu Catholic University, Daegu, Korea.
³Department of Food Science and Engineering, Daegu University, Daegu, Korea.

Abstract

PURPOSE
Cellulose is a natural substance from plants or bacteria. It is known that bacterial synthesized cellulose has an effect of wound healing. The aim of this study is to show the effect of bacterial synthesized cellulose from citrus on wound healing. METHODS: Three full-thickness skin defects were made on the back of Sprague-Dawley rats. Three wounds were treated by vaseline gauze(Group V), Algisite M(R)(Group A) and bacterial synthesized cellulose from citrus(Group C) was used for dressing on skin defect on rats. We analyzed the gross, histological and biochemistry finding.
RESULTS
Group C showed more decrease of wound size compared to Group V(33% versus 7.2#) after 14 days. The histologic findings revealed Group C and Group A preceed the process of wound healing rather than Group V(More rapid collagen deposition and neovascularization and reduced inflammation). Also, the expressions of vascular endothelial growth factor(VEGF) and transforming growth factor(TGF)-beta1 were increased in the Group C and Group A compared with the Group V in 7 days. VEGF and TGF-beta1 expression were decreased in the Group C and Group A in 14 days, however Group V was not decreased at 14 day because of delayed wound healing process.
CONCLUSION
Bacterial synthesized cellulose from citrus affects wound healing by reducing the inflammatory stage. And stimulates wound contracture by the deposition of extracellular matrix, thus preventing the formation of chronic wounds.

Keywords: Bacteria; Cellulose; Citrus; Wound healing