Experimental Assessment of Hemostatic Agents: Comparison with New Developed Chitosan-Based Material.

Cho, Young Kyoo; Lee, Sang Yun; Kim, Tae Jung; Lim, Hyun Ju; Oh, Eun Jung; Lee, Soo Bok; Choi, Kang Young; Yang, Jung Dug; Cho, Byung Chae; Chung, Ho Yun

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2011;38(4):369-375.
Published online July 1, 2011.

Experimental Assessment of Hemostatic Agents: Comparison with New Developed Chitosan-Based Material.

Young Kyoo Cho, Sang Yun Lee, Tae Jung Kim, Hyun Ju Lim, Eun Jung Oh, Soo Bok Lee, Kang Young Choi, Jung Dug Yang, Byung Chae Cho, Ho Yun Chung

¹Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyungpook National University, Daegu, Korea. hy-chung@knu.ac.kr
²Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu, Korea.
³Fiber & Tech Co., Gyeonggi-do, Korea.

Abstract

PURPOSE
Many hemostatic agents and dressings have been tested with variable degree of success. Chitosan has a positive charge, it attracts red blood cells, which have a negative charge. Our goal is to test the efficacy of new developed chitosan-based hemostatic materials in providing durable hemostasis in a high-flow arterial wound model. METHODS: We compared each group with SD rats motality tests and in vitro blood compatibility test by blood clotting index (BCI). We devided the SD rats into 6 groups (N =15) by type of hemostatic agents. A: 100% nonwoven chitosan (degree of the deacetylation: 90%). B: 50% N-acetylation on nonwoven of chitosan gel (degree of the deacetylation: 50%). C: 60% N-acetylation on nonwoven of chitosan ge (degree of the deacetylation: 40%)l. D: Cutanplast(R). E: HemCon(R) F: Gauze. In vivo test, a proximal arterial injury was created in unilateral femoral arteries of 90 anesthetized SD rats. Each materials was made same size and thickness then applied to the injury site for 3 minutes. In vitro test, we compared each group with BCI in human blood.
RESULTS
In vivo test, group A showed lower motality rate of 46% than any other groups, Group B and C showed lower motality rate of 60% than group D and E's motality rate of 66%. In vitro test, BCI of group A (30.6 +/- 1.2) and B (29.3 +/- 1.0) were showed nearly about group D (29.1 +/- 1.8) and E (27.4 +/- 1.6). Group C (37.1 +/- 2.0) showed higher BCI than group A and B, it means group C decreased blood clotting.
CONCLUSION
In conclusion, this study suggests a newly developed chitosan-based hemostatic materials induced durable hemostasis and increased blood clotting, and are considered as effective biologic hemostatic agents.

Keywords: Hemostatic agents; Chitosan; Blood clotting