Acceleration of Wound Healing Using Adipose-derived Stem Cell Therapy with Platelet Concentrates: Platelet-rich Plasma (PRP) vs. Platelet-rich Fibrin (PRF).

Hahn, Hyung Min; Jeon, Yeo Reum; Rha, Dong Kyun; Lew, Dae Hyun

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2011;38(4):345-350.
Published online July 1, 2011.

Acceleration of Wound Healing Using Adipose-derived Stem Cell Therapy with Platelet Concentrates: Platelet-rich Plasma (PRP) vs. Platelet-rich Fibrin (PRF).

Hyung Min Hahn, Yeo Reum Jeon, Dong Kyun Rha, Dae Hyun Lew

Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Korea. dhlew@yuhs.ac

Abstract

PURPOSE
Although platelet-rich plasma (PRP) potentiate the wound healing activity of adipose-derived stem cells (ADSCs), its effect cannot be sustained for a prolonged period of time due to short duration of action. This led us to design and produce platelet-rich fibrin (PRF), in an effort to develop a tool which lasts longer, and apply it on wound healing. METHODS: Two symmetrical skin defects were made on the back of seven nude mice. ADSCs were applied to each wound, combined with either PRP or PRF. The wound area was measured over 14 days. By day 16, the wound was harvested and histologic analysis was performed including counting of the blood vessel.
RESULTS
The healing rate was more accelerated in PRP group in the first 5 days (p<0.05). However, PRF group surpassed PRP group after 6 days (p<0.05). The average number of blood vessels observed in the PRF group was 6.53 +/- 0.51, compared with 5.68 +/- 0.71 for the PRP group.
CONCLUSION
PRF exerts a slow yet pervasive influence over the two-week course of the wound healing process. Thus, PRF is probably more beneficial for promoting the activity of ADSCs for a sustained period of time.

Keywords: Adipose-derived stem cell; Platelet-rich plasma; Platelet-rich fibrin; Wound healing