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Journal of the Korean Society of Plastic and Reconstructive Surgeons 2011;38(2):131-134.
Published online March 1, 2011.
Role of Reactive Oxygen Species in the Adipogenesis of Adipose-derived Stem Cells.
Hak Chang, Kyung Hee Min, Young In Park, Yo Han Kim, Kyung Won Minn
1Research Institute of Plastic and Reconstructive Surgery, Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, Korea.
2Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Seoul, Korea. mkh797@hanmail.net
Stem cells continue to receive research attention in the clinical fields, and adipose-derived stem cells(ADSCs) have been shown to be a good source raw material. Many plastic surgeons are researching the ADSC adipogenesis with a view of conducting clinical trials, and many attempts have been made to identify the factors that promote the adipogenesis of ADSCs, but comparatively few correlation studies have been undertaken to explore the relation between reactive oxygen species(ROS) and the ADSC adipogenesis. We undertook this study is to investigate the effects of ROS on ADSC adipogenesis. METHODS: ADSCs were isolated and cultured from abdominal adipose tissue, and cultured in different media; 1) DMEM(control), 2) adipogenesis induction culture medium, 3) adipogenesis induction culture medium with ROS(20 microM/50 microM H2O2), 4) adipogenesis induction culture medium containing ROS(20 microM/50 microM H2O2) and antioxidant(10 microM/20 microM Deferoxamine). We compared adipogenesis in these different media by taking absorbance measurements after Oil-Red O staining every 5 days.
After culturing for 20 days, significant differences were observed between these various culture groups. Absorbance results showed significantly more adipogenesis had occurred in media containing adipogenesis induction culture medium and H2O2(in a H2O2 dose-dependently manner) than in media containing adipogenesis induction culture medium and no H2O2(p<0.001). Furthermore, in media containing adipogenesis induction culture medium, H2O2, and antioxidant, absorbance results were significantly lower than in adipogenesis induction culture medium and H2O2(p<0.001).
These findings suggest that ROS promote the adipogenesis of ADSCs. We suggests that ROS could be used in the adipose tissue engineering to improve fat cell differentiation and implantable fat tissue organization.
Keywords: Reactive oxygen species(ROS); Adipose-derived stem cells(ADSCs); Adipogenesis
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