Effect of Relaxin Expressing Adenovirus for Rat Skin Flap Viability.

Yun, In Sik; Park, Yong Sun; Cheon, Young Woo; Jeon, Yeo Reum; Lee, Won Jai; Yun, Chae Ok; Rah, Dong Kyun

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Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2010;37(5):519-525.
Published online September 1, 2010.

Effect of Relaxin Expressing Adenovirus for Rat Skin Flap Viability.

In Sik Yun, Yong Sun Park, Young Woo Cheon, Yeo Reum Jeon, Won Jai Lee, Chae Ok Yun, Dong Kyun Rah

¹Institue for Human Tissue Restoration & Department of Plastic & Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Korea. dkrah@yuhs.ac
²Yonsei Cancer Research Center, Yonsei University College of Medicine, Seoul, Korea.

Abstract

PURPOSE
Of various effects of relaxin, we assumed that anti-fibrotic effects, neovascularization effects and vasodilatation effects of relaxin might enhance the survival rate of skin flap. In the current study, we used adenovirus expressing relaxin genes to examine whether these genes could enhance the survival rate of a skin flap. METHODS: A total of 30 Sprangue-Dawley rats were divided into three groups: RLX group (10; relaxin virus injected group), CTR group (10; no gene coded virus injection group), and PBS group (10; PBS injected group). Each group was intradermally injected with the virus (107 PFU) and PBS 48 hours before and immediately before the flap elevation. A distally based flap 3 x 9 cm in size was elevated on the dorsal aspect of each rat. Following this, a flap was placed in the original location and then sutured using a #4-0 Nylon. A surviving area of the flap was measured and then compared on postoperative days 3, 7 and 10. Using a laser Doppler, the amount of blood flow was measured. On postoperative day 10, tissues were harvested for histologic examination and the number of blood vessels was counted.
RESULTS
There was a significant increase in the area of the flap survival in the RLX group on postoperative days 3 and 7. The Doppler measurement also showed significantly increased blood flow immediately after the operation and on postoperative days 7 and 10. The number of blood vessels was significantly greater in the RLX group in the tissue harvested on postoperative day 10. The VEGF concentration was significantly higher in the RLX group than others in the tissues harvested on postoperative day 10.
CONCLUSION
Following an analysis of the effects of relaxin-secreting adenovirus on the survival of a flap, the surviving area of the flap and the blood flow also increased. A histopathology also showed an increase in the number of blood vessels and the concentration of VEGF.

Keywords: Relaxin; Adenovirus; Gene therapy; Angiogenesis; Flap

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