Difference of Gene Expression between Hypertrophic Scar Keratinocytes and Normal Keratinocytes.

Choi, Sung Won; Chung, Ho Yun; Lim, Young Kook; Kim, Hoon Nam; Oh, Ji Won; Kim, Moon Kyu; Jeon, Sae Hwa; Hong, Yong Taek

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Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2010;37(4):317-322.
Published online July 1, 2010.

Difference of Gene Expression between Hypertrophic Scar Keratinocytes and Normal Keratinocytes.

Sung Won Choi, Ho Yun Chung, Young Kook Lim, Hoon Nam Kim, Ji Won Oh, Moon Kyu Kim, Sae Hwa Jeon, Yong Taek Hong

¹Department of Plastic and Reconstructive Surgery, Fatima Hospital, Daegu, Korea. plastika@hanmail.net
²Department of Plastic and Reconstructive Surgery, School of Medicine, Kyngpook National University, Daegu, Korea.
³Department of Immunology, School of Medicine, Kyngpook National University, Daegu, Korea.
⁴Tego Science Inc, Daegu, Korea.

Abstract

PURPOSE
There is no clear evidence of the original cause of hypertrophic scar, and the effective method of treatment is not yet established. Recently the steps of searching in gene and molecular level are proceeding. we are trying to recognize the difference between keratinocytes of hypertrophic scar and normal skin. Then we do support the comprehension of the scar formation mechanism and scar management. METHODS: Total RNAs were extracted from cultured keratinocytes from 4 hypertrophic scars and normal skins. The cDNA chips were prepared. A total of 3063 cDNAs from human cDNA library were arrayed. And the scanning data were analyzed.
RESULTS
On microarray, heat shock protein, pyruvate kinase, tumor rejection antigen were more than 2 fold intensity genes. Among them, heat shock 70 kd protein showed the strongest intensity difference.
CONCLUSION
In this study, it can be concluded that heat shock proteins play an important role in the process of wound healing and scar formation. This study provides basic biologic information for scar research. The new way of the prevention and treatment of scar formation would be introduced with further investigations.

Keywords: Hypertrophic scar; Keratinocyte; Heat shock protein

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