Vascular Endothelial Growth Factor Effect on Notch 1 Expression and Proliferation of Fibroblast.

Koh, Sung Hoon

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2010;37(1):7-11.
Published online January 1, 2010.

Vascular Endothelial Growth Factor Effect on Notch 1 Expression and Proliferation of Fibroblast.

Department of Plastic & Reconstructive Surgery, Hallym University Sacred Heart Hospital, Gyeonggi-do, Korea. pshkoh@hanmail.net

Abstract

PURPOSE
Vascular endothelial growth factor (VEGF) is known as a growth factor of endothelium and fibroblast. The purpose is to know the VEGF effects on fibroblast proliferation and fibroblast's notch receptor expression. METHODS: CCD-986sk fibroblast was purchased from the Korean Cell Bank and was used in XTT assay for proliferation and wound healing assay for migration. Immunofluorescent (IF) staining and western blotting were used in testing notch expression of fibroblast. Semiquantitative RT-PCR was used in checking notch 1 mRNA production by fibroblast. Student-t test was used for analyzing results.
RESULTS
Cell proliferation assay using XTT showed significant higher proliferation in VEGF treated fibroblast, 2.324 +/- 0.0026 vs. 2.463 +/- 0.017 (p=0.002). Wound healing assay showed longer migration in VEGF treated fibroblast (p=0.062). The fluorescence was brighter in VEGF treated cells of notch 1 IF staining. Notch 1 expressions and mRNA productions increased more in VEGF treated cells.
CONCLUSION
VEGF stimulates fibroblast to proliferate, migrate and to express Notch 1 simultaneously. Notch receptor could be related to VEGF mediated wound healing.

Keywords: Fibroblast; Notch receptor; Vascular endothelial growth factor