Clinical Considerations on Contamination Rates of Cryopreserved Autologous Fat.

Kim, Jeong Tae; Seo, Woo Jin; Kim, Yeon Hwan

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Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2009;36(6):685-690.
Published online November 1, 2009.

Clinical Considerations on Contamination Rates of Cryopreserved Autologous Fat.

Jeong Tae Kim, Woo Jin Seo, Yeon Hwan Kim

Department of Plastic and Reconstructive Surgery, College of Medicine, Hanyang University, Seoul, Korea. jtkim@hanyang.ac.kr

Abstract

PURPOSE
Autologous fat graft is a widely accepted technique used for soft tissue augmentation. Nonetheless, the use of fat graft is limited due to unpredictable survival rates and repeated grafting. To avoid repeated grafting, cryopreserved fat graft technique has recently been widely used. On the other hand, the number of patients with chronic infection (who received cryopreserved fat injection) has currently been increasing. This study is focused on the safety of cryopreserved fat injection from the infection. METHODS: We collected 150 samples from local aesthetic clinics to examine the safety of cryopreserved autologous fat. To test for microbacterial contaminations of the cryopreserved fat specimens, microbacterial cultures & antibiotics sensitivity tests were performed. Then, we examined possible correlation between the preservation period and donor sites, focused on the results of microbacterial culture.
RESULTS
Cultures were positive for Staphylococcus epidermidis in 5 samples (methicillinresistant Staphylococcus epidermidis in 4 samples), Micrococcus species in 3 samples. An average duration of preservation was 191 days and there found no significant correlation between the duration of preservation and microbacterial growth.
CONCLUSION
Staphylococcus epidermidis was the leading cause of cryopreserved fat contamination, and the resistance to methicillin is common. Based on the above results, aseptic handling of fat during harvesting and preservation appeared to be most important.

Keywords: Cryopreservation; Adipose tissue; Focal infection

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