Effective Delivering Method of Umbilical Cord Blood Stem Cells in Cutaneous Wound Healing.

Park, Sang Eun; Han, Seung Bum; Rah, Dong Kyun; Lew, Dae Hyun

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2009;36(5):519-524.
Published online September 1, 2009.

Effective Delivering Method of Umbilical Cord Blood Stem Cells in Cutaneous Wound Healing.

Sang Eun Park, Seung Bum Han, Dong Kyun Rah, Dae Hyun Lew

Institute for Human Tissue Restoration, Department of Plastic & Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Korea. dhlew@yuhs.ac

Abstract

PURPOSE
This study was conducted to identify the most effective method in cell therapy by comparing and analyzing the level of wound healing after the application of various cell delivery methods. METHODS: Human mesenchymal stem cells were administered using 5 different methods on full thickness skin defects which were deliberately created on the back of 4-week-old mice using a 8 mm punch. Different modes of administration, cell suspension, local injection, collagen GAG matrix seeding, fibrin, and hydrogel mix methods were used. In each experiment group, 4x10(5) mesenchymal stem cells were administered according to 5 deferent methods, except for the corresponding control group.
RESULTS
The wound healing rate was fastest in the local injection group. The wound healing rate was relatively slow in the collagen matrix group, however, the number of blood vessels or VEGF increased most in this group.
CONCLUSION
For rapid wound healing through wound contraction, it is advantageous to administer MSC by the local injection method. For the healing process of a wide area, such as a burn, the seeding of cells to collagen matrix is thought to be effective.

Keywords: Tissue therapy; Wound healing; Mesenchymal stem cell