J Korean Soc Plast Reconstr Surg Search

CLOSE


Journal of the Korean Society of Plastic and Reconstructive Surgeons 2009;36(1):19-23.
Published online January 1, 2009.
Optimal Concentration of Thrombin to Activate Platelet for Wound Healing.
Soo Jin Eum, Seung Kyu Han, Kyung Wook Chun, Woo Kyung Kim
Department of Plastic Surgery Korea University College of Medicine, Seoul, Korea. pshan@kumc.or.kr
Abstract
PURPOSE
Platelet transplantation is a novel therapeutic strategy for acceleration of wound healing. When applying platelets, efficacy of adding thrombin to stimulate growth factor release from platelets has already been proven. However, no quantitative data of the thrombin treatment has been reported. The purpose of this study is to determine the optimal thrombin concentration to maximize growth factor release of platelets. In particular, this study was designed to quantify levels of platelet derived growth factor(PDGF)-BB, which is a major growth factor containing in the platelets, in vitro. METHODS: Fresh platelets were obtained from a blood bank. They were suspended in DMEM/F-12 and incubated with thrombin of various concentrations. The concentrations of thrombin tested were 0, 6.25, 12.5, 25, 50, 100, 200, and 400IU/mL. After 30 minutes, 1, 3, 5, and 7 days, the levels of PDGF-BB were measured using enzyme linked immunosorbent assay. Platelets from four donors were included in this study. Each sample was tested in triplicate and the mean value was used as a data for each sample.
RESULTS
The addition of thrombin increased the level of PDGF-BB. Increases in storage time of platelets resulted in decreased levels of PDGF-BB. Higher levels of PDGF were detected in consort with increased thrombin concentrations. However, there was no significant difference between samples of 200 and 400IU/mL concentrations.
CONCLUSION
The results indicate that adding thrombin accelerates the release of growth factors from platelets and the optimal thrombin concentration to maximize this function is 200IU/mL.
Keywords: Platelet; Wound healing; Thrombin
TOOLS
Share :
Facebook Twitter Linked In Google+ Line it
METRICS Graph View
  • 1,193 View
  • 0 Download

Sequential appearance and distribution of fibronectin in granulation tissue and wound healing.1991 November;18(6)



ABOUT
ARTICLE & SPECIALITY
Article category

Browse all articles >

Speciality

Browse all articles >

BROWSE ARTICLES
AUTHOR INFORMATION
Editorial Office
101-2003, Lotte Castle President, 109, Mapodaero, Mapo-gu, Seoul 04146, Korea
E-mail: apsedit@gmail.com                

Copyright © 2024 by Korean Society of Plastic and Reconstructive Surgeons.

Developed in M2PI

Close layer
prev next