The Effects of Human Adipose Tissue-derived Stem Cells on Degenerative Change of Knee in Rabbit Model.

Jeong, Ki Hwan; Kim, Seok Kwun; Jeong, Jae Oo; Heo, Jeong; Kwon, Yong Seok; Lee, Keun Cheol

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2008;35(6):637-644.
Published online November 1, 2008.

The Effects of Human Adipose Tissue-derived Stem Cells on Degenerative Change of Knee in Rabbit Model.

Ki Hwan Jeong, Seok Kwun Kim, Jae Oo Jeong, Jeong Heo, Yong Seok Kwon, Keun Cheol Lee

Department of Plastic & Reconstructive Surgery, College of Medicine. Dong-A University, Busan, Korea

Abstract

PURPOSE
The survival of bone marrow derived stem cell was reported several times. But the survival of adipose tissue derived stem cells(hASCs) was not mentioned on. We studied the adipose tissue derived stem cell's survival and effect on articular cartilage in rabbits. METHODS: Osteoarthritis was induced in twenty New Zealand white rabbits by intraarticular injection of monosodium iodoacetate(MIA). After four weeks, hASCs were also injected into the knee joints space without any vehicle, but the control group received phosphate buffered saline only. The histologic grade of articular cartilage was measured in 4 and 8 weeks after the transplantation of hASC and the viability of injected stem cells measured by Fluorescent in situ Hybridization (FISH) examination.
RESULTS
After 4 and 8 weeks from hASCs transplantation, histologic grade was not significantly difference between two groups(p>0.05), and the Y chromosome of the transplanted hASCs was not detected in articular cartilage.
CONCLUSION
We found that direct injection of hASC in joint space didn't work on damaged articular cartilage repair.

Keywords: hASCs; Stem cell; Osteoarthritis