Effect of Oncostatin M on Wound Healing Activity of Diabetic Fibroblasts in vitro.

Lim, Hyung Woo; Chun, Kyung Wook; Han, Seung Kyu; Kim, Woo Kyung

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2008;35(4):355-359.
Published online July 1, 2008.

Effect of Oncostatin M on Wound Healing Activity of Diabetic Fibroblasts in vitro.

Hyung Woo Lim, Kyung Wook Chun, Seung Kyu Han, Woo Kyung Kim

Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea. pshan@kumc.or.kr

Abstract

PURPOSE
Oncostatin M(OSM) has been known as a role in fibrosis and anti-inflammatory effects of various organs and tissues. Although there have been a number of studies which are focused on the roles and mechanisms of OSM, there are few reports on its effects in chronic wound healing. The purpose of this study is to evaluate the effects of OSM in wound healing activities of dermal fibroblasts of chronic wound in vitro. In particular, this study is focused on cell proliferation and synthesis of collagen and glycosaminoglycan(GAG), which are the major components of the extracellular matrices, of diabetic fibroblasts. METHODS: Fibroblasts were isolated from excess skin that was obtained from diabetic foot ulcer patients who underwent debridement. The isolated fibroblasts were cultivated in presence of OSM(100ng/mL). Cell proliferation, collagen synthesis and GAG levels were compared.
RESULTS
All the components tested in this study increased in OSM treatment group. In particular, collagen and GAG synthesis demonstrated statistically significant increases(p<0.05 in the Mann-Whitney U- test).
CONCLUSION
These results indicate that OSM increases wound healing activities of dermal fibroblasts of chronic wound in vitro.

Keywords: Oncostatin M; Diabetic fibroblasts